Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | has a regulatory role in pentaose phosphate pathway | Canis lupus familiaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics, rapid equilibrium random bi bi kinetic model | Canis lupus familiaris | |
0.01 | - |
NADP+ | pH 7.4, 37°C, with D-glucose 6-phosphate or 2-deoxy-D-glucose 6-phosphate | Canis lupus familiaris | |
0.122 | - |
D-glucose 6-phosphate | pH 7.4, 37°C, with NADP+ or deamino-NADP+ | Canis lupus familiaris | |
2 | 3 | deamino-NADP+ | pH 7.4, 37°C, with D-glucose 6-phosphate | Canis lupus familiaris | |
24 | - |
2-deoxy-D-glucose 6-phosphate | pH 7.4, 37°C, with NADP+ | Canis lupus familiaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, can substitute for Mn2+ | Canis lupus familiaris | |
Mn2+ | required, can substitute for Mg2+ | Canis lupus familiaris | |
additional information | high ionic strength inactivates the enzyme | Canis lupus familiaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
native PAGE | Canis lupus familiaris |
52500 | - |
2 * 52500, SDS-PAGE | Canis lupus familiaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD(P)+ | Canis lupus familiaris | key enzyme catalyzing the first step of the pentose phosphate pathway generating NADPH for anabolic metabolsim pathways and protection systems in the liver | D-glucono-1,5-lactone 6-phosphate + NAD(P)H | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2000fold by 2times 2',5'-ADP affinity chromatography and gel filtration | Canis lupus familiaris |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | rapid equilibrium random bi bi kinetic mechanism | Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Canis lupus familiaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
130 | - |
purified enzyme | Canis lupus familiaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose 6-phosphate + NADP+ | - |
Canis lupus familiaris | 2-deoxy-D-glucono-1,5-lactone 6-phosphate + NADPH | - |
? | |
D-glucose 6-phosphate + deamino-NADP+ | - |
Canis lupus familiaris | D-glucono-1,5-lactone 6-phosphate + deamino-NADPH | - |
? | |
D-glucose 6-phosphate + NAD(P)+ | key enzyme catalyzing the first step of the pentose phosphate pathway generating NADPH for anabolic metabolsim pathways and protection systems in the liver | Canis lupus familiaris | D-glucono-1,5-lactone 6-phosphate + NAD(P)H | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Canis lupus familiaris | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52500, SDS-PAGE | Canis lupus familiaris |
More | low pH value and low ionic strength cause aggregation of the enzyme in active oligomeric forms, e.g. as tetramer or hexamer | Canis lupus familiaris |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Canis lupus familiaris |
glucose-6-phosphate dehydrogenase | - |
Canis lupus familiaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Canis lupus familiaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Canis lupus familiaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
high pH value inactivates the enzyme | Canis lupus familiaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
deamino-NADP+ | not as effective as NADP+ | Canis lupus familiaris | |
NADP+ | - |
Canis lupus familiaris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
NADPH | pH 7.4, 37°C | Canis lupus familiaris |